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Related Experiment Videos

Anion effects on pyruvate kinase.

P J Taylor, J L Robinson

    The Biochemical Journal
    |October 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Rabbit muscle pyruvate kinase is not activated by common anions like acetate or chloride when tested. This finding challenges previous research conclusions regarding enzyme activation by these substances.

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    Area of Science:

    • Biochemistry
    • Enzymology

    Background:

    • Pyruvate kinase (PK) is a key enzyme in glycolysis.
    • Anion activation of muscle pyruvate kinase has been previously reported.

    Purpose of the Study:

    • To investigate the effect of various anions on rabbit muscle pyruvate kinase activity.
    • To re-evaluate previous findings on anion-mediated enzyme activation.

    Main Methods:

    • Purification of rabbit muscle pyruvate kinase.
    • Enzyme activity assays using tetramethylammonium salts of acetate, chloride, lactate, nitrate, and sulphate.

    Main Results:

    • Rabbit muscle pyruvate kinase showed no activation by acetate, chloride, lactate, nitrate, or sulphate anions.
    • Results were obtained using tetramethylammonium salts to avoid confounding effects of other cations.

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    Conclusions:

    • The tested anions do not activate rabbit muscle pyruvate kinase.
    • This study contradicts prior conclusions by Focant & Watts (1973) regarding anion activation.