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Updated: Aug 9, 2026

Clonogenic Assay: Adherent Cells
Published on: March 13, 2011
On the theory of clonal selection in carcinogenic transformation
Abstract:
A temperature sensitive variant (ts13) of African green monkey kidney cell line, carrying a temperature sensitive lesion in thymidine metabolism, was transformed by 40 μg/ml of methylnitrosourea (MNU) at the restricted temperature of 39·5°C, whereas the same cell line was not transformed by MNU at the permissive temperature of 33°C. Results presented in this paper raise the possibility that clones of cells carrying a biochemical lesion might possibly contribute to carcinogenesis with respect to certain chemical carcinogens.
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