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Related Experiment Videos

Homologous interference induced by Sindbis virus.

R E Johnston, K Wan, H R Bose

    Journal of Virology
    |November 1, 1974
    PubMed
    Summary

    Prior Sindbis virus infection reduces progeny virus yields by limiting the number of infected cells. This homologous interference requires an active viral genome and parallels virus attachment kinetics.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Cell Biology

    Background:

    • Homologous interference is a phenomenon where prior infection with a virus reduces the yield of a superinfecting virus of the same type.
    • Sindbis virus is a well-characterized alphavirus used to study viral replication and interference mechanisms.

    Purpose of the Study:

    • To investigate the mechanisms underlying homologous interference during Sindbis virus infection.
    • To determine the role of the viral genome and specific viral mutants in the development of interference.

    Main Methods:

    • Infection of chicken embryo fibroblast and BHK(21) cell cultures with Sindbis virus.
    • Analysis of progeny virion yields following superinfection.
    • Assessment of viral genome activity using ultraviolet light inactivation and temperature-sensitive mutants (ts-24, ts-6, ts-2, ts-20).

    Main Results:

    • Prior Sindbis virus infection significantly reduced progeny virion yields of the superinfecting genotype.
    • Interference was attributed to a decrease in the number of cells capable of producing the superinfecting virus.
    • The development of interference correlated with Sindbis virus attachment kinetics.
    • Interference was dependent on an active viral genome, as evidenced by sensitivity to UV inactivation and the failure of RNA(-) mutant ts-24 to induce interference under nonpermissive conditions.
    • RNA(-) mutant ts-6 and RNA(+) mutants ts-2 and ts-20 demonstrated interference at both permissive and nonpermissive temperatures.

    Conclusions:

    • Homologous interference in Sindbis virus infection is mediated by a reduction in the number of permissive cells.
    • An intact and active viral genome is essential for the induction of interference.
    • Specific viral mutants exhibit differential behavior regarding interference, providing insights into the underlying molecular mechanisms.

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