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Phenol sulfotransferases.

R D Sekura, W B Jakoby

    The Journal of Biological Chemistry
    |July 10, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Researchers purified two rat liver phenol sulfotransferases, crucial for detoxifying phenolic compounds. These enzymes conjugate xenobiotics and may produce indican from tryptophan metabolism.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Toxicology

    Background:

    • Phenol sulfotransferases (PSTs) play a vital role in the metabolism and detoxification of phenolic compounds.
    • Understanding the properties and functions of PSTs is crucial for comprehending xenobiotic conjugation and endogenous metabolite production.

    Purpose of the Study:

    • To purify and characterize two distinct phenol sulfotransferases from rat liver.
    • To investigate the substrate specificity and kinetic properties of the purified enzymes.
    • To elucidate the potential in vivo roles of these enzymes in detoxification and metabolism.

    Main Methods:

    • Conventional protein purification techniques.
    • Affinity chromatography using Affi-Gel blue and ATP-agarose.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for homogeneity assessment.

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  • Enzyme kinetics and substrate specificity assays using adenosine 3'-phosphate 5'-phosphosulfate (PAPS).

    • Main Results:

    • Two homogeneous phenol sulfotransferases were purified from rat liver.
    • Both enzymes exhibit a molecular weight of approximately 65,000 Da, composed of two equal subunits.
    • The enzymes share similar substrate specificity and kinetic parameters, accepting various phenolic compounds but not sterols, alcohols, bile acids, or hydroxamates.
    • Differences were observed in amino acid composition and elution profiles from DEAE-cellulose.
    • Enzymes catalyzed the formation of indican from 3-hydroxyindole.

    Conclusions:

    • Rat liver contains at least two distinct phenol sulfotransferases with overlapping but distinct properties.
    • These enzymes function as detoxification enzymes, conjugating xenobiotics and endogenous phenolic compounds.
    • The ability to convert 3-hydroxyindole to indican suggests a role in tryptophan metabolism.