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Updated: Aug 16, 2026

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell
Published on: October 22, 2014
Transport of catecholamines by resealed chromaffin-grnaule "ghosts"
Abstract:
A method is described for the preparation of resealed chromaffin-granule ;ghosts'. The lysis and rapid purification procedures provide ;ghosts' in approximately 70% yield from crude granules; the preparation contains 0.1mumol of catecholamine/mg of protein (as compared with 2.8mumol/mg in unlysed granules), of which about one third is inside the ;ghosts'. The ;ghosts' retain their ability to accumulate catecholamines, a process dependent on Mg-ATP and inhibited by reserpine, and a simple assay for this transport is described.
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