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A solid-phase radioimmunoassay for plasma progesterone.

K K Dighe, W M Hunter

    The Biochemical Journal
    |October 1, 1974
    PubMed
    Summary
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    This study presents a precise plasma progesterone assay using microcrystalline cellulose-linked antiserum. The method offers high sensitivity and accuracy for tracking progesterone levels throughout the menstrual cycle.

    Area of Science:

    • Endocrinology
    • Biochemistry
    • Assay Development

    Background:

    • Accurate measurement of plasma progesterone is crucial for understanding reproductive physiology.
    • Existing assay methods may have limitations in precision, sensitivity, or practicality.

    Purpose of the Study:

    • To develop and validate a novel, precise, and sensitive assay for plasma progesterone.
    • To enable accurate monitoring of progesterone levels across the menstrual cycle.

    Main Methods:

    • A routine assay utilizing antiserum covalently linked to microcrystalline cellulose.
    • Comparison with the double-antibody method as a reference separation system.
    • Single-step extraction with light petroleum after ethanol addition to plasma.

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    Main Results:

    • The developed assay demonstrates high precision and sensitivity, comparable to the double-antibody method.
    • Consistent recovery rates (92.4+/-1.2%) of added [(3)H]progesterone were achieved, simplifying sample processing.
    • The assay effectively measures progesterone in small plasma volumes (200 μL), allowing detection of early periovulatory rises.
    • Progesterone-like activity was detected in urine, with a fourfold increase in excretion during the luteal phase.

    Conclusions:

    • The microcrystalline cellulose-linked antiserum assay provides a robust and sensitive method for plasma progesterone determination.
    • This assay facilitates detailed monitoring of the menstrual cycle and associated hormonal changes.
    • The findings highlight the utility of this assay in both clinical and research settings for reproductive hormone analysis.