Inhibition of mitochondrial phosphate transport by p-diazobenzenesulphonate

The impermeant protein-modifying reagent p-diazobenzenesulphonate inhibits phosphate transport in rat liver mitochondria. The rate of inhibition is increased by the presence of oxidizable substrate, and decreased by uncoupling agents. These results are compared with the results on inhibition of phosphate transport by N-(N-acetyl-4-sulphamoylphenyl)maleimide (Klingenberg et al., 1974).