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Updated: Oct 14, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
Protein electrophoretic profiles and the origin of the B genome of wheat
Abstract:
Protein electrophoretic profiles cast doubt upon the prevalent theory that the B genome of the polyploid wheats was derived from a species of Aegilops. They suggest, instead, that the wild tetraploid wheats comprise a complex, whose components were derived from various combinations of diploid Triticum types, which evidently include the B-genome type.
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Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Two-dimensional Gel Electrophoresis
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Biological samples, such as cells...