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Related Experiment Videos

Sugar effects on murine sarcoma virus transformation.

M Hatanaka

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1973
    PubMed
    Summary

    Carbohydrates like D-mannose affect murine sarcoma virus transformation. Specific sugars repressed hexose uptake in normal mouse cells, altering tumor formation and viral transformation.

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    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Murine sarcoma virus (MSV) infection leads to enhanced hexose uptake in transformed cells.
    • The role of specific carbohydrates in modulating viral transformation and cellular metabolism is not fully understood.

    Purpose of the Study:

    • To investigate the influence of different carbohydrates in culture media on MSV-induced fibroblast transformation.
    • To elucidate the mechanisms by which carbohydrates affect hexose uptake and viral transformation.

    Main Methods:

    • NIH Swiss mouse fibroblasts were cultured in media supplemented with various carbohydrates (D-mannose, 2-deoxy-D-glucose, D-xylose, L-xylose, L-arabinose, D-galactose).
    • Assessed the number of transformed colonies in vitro and tumor formation in vivo.
    • Measured the uptake of D-glucose, D-mannose, and D-galactose in normal and transformed cells under different carbohydrate conditions.

    Main Results:

    • D-mannose and 2-deoxy-D-glucose reduced the number of transformed colonies and tumor formation.
    • These sugars repressed hexose uptake in normal mouse cells, interfering with the characteristic enhancement seen in MSV-infected cells.
    • D-xylose, L-xylose, and L-arabinose derepressed hexose uptake, while D-galactose had an intermediate effect.
    • MSV transformation consistently enhanced hexose uptake beyond levels observed in derepressed control cells, indicating an additional viral mechanism.

    Conclusions:

    • The type of carbohydrate in the culture medium significantly influences MSV-induced transformation.
    • Specific carbohydrates can interfere with viral transformation by modulating hexose uptake pathways.
    • MSV infection employs distinct mechanisms to enhance hexose uptake, independent of cellular metabolic states induced by external sugars.

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