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UV Irradiation: A Method to Integrate Extrachromosomal Arrays03:40

UV Irradiation: A Method to Integrate Extrachromosomal Arrays

Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene.  UV Irradiation can induce chromosomal integration of the array and more stable transgene...
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Intestinal Epithelial Regeneration in Response to Ionizing Irradiation09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

The gastrointestinal tract is one of the most sensitive organs to injury upon radiotherapeutic cancer treatments. It is simultaneously an organ system with one of the highest regenerative capacities following such insults. The presented protocol describes an efficient method to study the regenerative capacity of the intestinal...
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Laser Micro-Irradiation to Study DNA Recruitment During S Phase07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser...
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Growth and Characterization of Irradiated Organoids from Mammary Glands09:49

Growth and Characterization of Irradiated Organoids from Mammary Glands

Organoids developed from mouse mammary glands were irradiated and characterized to assess epithelial traits and interactions with immune cells. Irradiated organoids can be used to better evaluate cell-cell interactions that may lead to tumor cell recruitment in irradiated normal...
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PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator10:48

PET and MRI Guided Irradiation of a Glioblastoma Rat Model Using a Micro-irradiator

In the past, small animal irradiation was usually performed without the ability to target a well-delineated tumor volume. The goal was to mimic the treatment of human glioblastoma in rats. Using a small animal irradiation platform, we performed MRI-guided 3D conformal irradiation with PET-based sub-volume boosting in a preclinical...
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A Mouse Model of Fatigue Induced by Peripheral Irradiation04:15

A Mouse Model of Fatigue Induced by Peripheral Irradiation

We describe a method using targeted peripheral irradiation to induce fatigue-like behavior in mice. The selected non-lethal irradiation dose leads to a week-long reduction in voluntary wheel-running...
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