Guanosine 5'-diphosphate, 3'-diphosphate: assignment of structure by 13C nuclear magnetic resonance spectroscopy

Guanosine tetraphosphate, recently discovered to mediate the regulatory relationship between protein synthesis and RNA accumulation in various bacteria, has been synthesized in vitro in large quantities and analyzed by natural-abundance (13)C nuclear magnetic resonance spectroscopy in order to confirm its structure and establish the positions of phosphate attachment. These studies have established its structure as guanosine 5'-diphosphate, 3'-diphosphate.