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Electron-microscopic study of measles virus in lymphocytes of affected children
Abstract:
Two different types of inclusion bodies have been found by electron-microscopy in the cytoplasm of sectioned lymphocytes from children infected with measles.The first is tubular, 18 nm. internal diameter, embedded in osmiophilic material, and is found during the incubation period. It is not thought to be specific for measles, and is probably identical with structures recently reported in HEp-2 cell lines and tumour tissue.The second type is less obviously tubular and morphologically resembles measles nucleocapsid. This was found only after the rash had appeared.
Insights
Electron microscopy revealed two distinct inclusion bodies in measles-infected children
Area of Science:
- Virology
- Cell Biology
- Pediatrics
Background:
- Measles virus infection can lead to characteristic cellular changes.
- Inclusion bodies are abnormal structures found within cells during viral infections.
- Understanding viral cytopathology aids in diagnosis and understanding disease progression.
Purpose of the Study:
- To characterize and differentiate inclusion bodies observed in lymphocytes of children with measles.
- To determine the temporal appearance and potential specificity of these inclusion bodies.
Main Methods:
- Electron microscopy was used to examine sectioned lymphocytes from children infected with measles.
- Morphological analysis was performed on identified inclusion bodies.
Main Results:
- Two distinct types of cytoplasmic inclusion bodies were identified in measles-infected lymphocytes.
- Type 1: Tubular, 18 nm internal diameter, embedded in osmiophilic material, observed during incubation period; not specific to measles.
- Type 2: Less tubular, resembling measles nucleocapsid, observed only after rash onset.
Conclusions:
- Distinct morphological forms of inclusion bodies are present in measles infection.
- The second type of inclusion body, resembling measles nucleocapsid, may serve as a more specific indicator of active measles replication.
- Further investigation into the nature and significance of these inclusion bodies is warranted.