Related Experiment Video

Updated: Aug 12, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

DNA polymerase 3 star requires ATP to start synthesis on a primed DNA

W Wickner, A Kornberg

    Proceedings of the National Academy of Sciences of the United States of America
    |December 1, 1973
    PubMed

    More Related Videos

    Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins08:14

    Kinetics of Lagging-strand DNA Synthesis In Vitro by the Bacteriophage T7 Replication Proteins

    Published on: February 25, 2017

    DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling08:04

    DNA Sequence Recognition by DNA Primase Using High-Throughput Primase Profiling

    Published on: October 8, 2019

    Related Concept Videos

    Proofreading01:43

    Proofreading

    Synthesis of new DNA molecules starts when DNA polymerase links nucleotides together in a sequence that is complementary to the template DNA strand. DNA polymerase has a higher affinity for the correct base to ensure fidelity in DNA replication. The DNA polymerase furthermore proofreads during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.Errors during Replication Are Corrected by the DNA Polymerase EnzymeGenomic DNA is synthesized in...
    PCR01:32

    PCR

    Overview
    DNA Replication02:40

    DNA Replication

    DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
    Replication in Prokaryotes
    DNA replication uses a large number of...
    Lagging Strand Synthesis01:59

    Lagging Strand Synthesis

    During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
    There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
    The Replisome03:01

    The Replisome

    DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
    The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
    Proofreading01:31

    Proofreading

    Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
    Errors During Replication are Corrected by the DNA Polymerase Enzyme