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Int-constitutive mutants of bacteriophage lambda.

K Shimada, A Campbell

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1974
    PubMed
    Summary
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    Constitutive trpB enzyme production in Escherichia coli requires a specific prophage site (p(I)). Mutations affecting phage gene regulation, particularly the int gene, influence this enzyme production.

    Area of Science:

    • Molecular Biology
    • Microbial Genetics

    Background:

    • Investigating the genetic regulation of enzyme production in Escherichia coli.
    • Examining the role of bacteriophage lambda (λ) prophage integration in host gene expression.

    Purpose of the Study:

    • To identify the specific prophage elements responsible for constitutive trpB enzyme production in a modified Escherichia coli strain.
    • To characterize mutations affecting phage gene regulation and their impact on enzyme synthesis.

    Main Methods:

    • Deletion analysis of the lambda prophage integrated within the trpC gene of Escherichia coli.
    • Mutagenesis and selection of lysogenic mutants exhibiting growth on low indole concentrations.
    • Characterization of phage gene derepression in selected mutants.

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    Main Results:

    • Enzyme production was dependent on a site (p(I)) located near the left end of the prophage.
    • Two classes of mutations were identified: v2-type (derepressing all leftward operator-controlled genes) and int-c type (derepressing only the int gene).
    • int-c mutations were localized to the p(I) region and exhibited a deficiency in xis gene function.

    Conclusions:

    • A specific prophage site (p(I)) is essential for constitutive trpB enzyme production.
    • Regulation of phage integrase (int) gene expression is critical for modulating host enzyme synthesis.
    • The xis gene product is not required for int-c mediated derepression of the int gene.