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Membrane excitability: made temperature-dependent by mutations.

Y Satow, S Y Chang, C Kung

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1974
    PubMed
    Summary
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    High temperatures disrupt action potential generation in Paramecium aurelia mutants. These findings suggest that multiple gene products, likely membrane proteins, are essential for cellular excitation.

    Area of Science:

    • Cellular biology
    • Genetics
    • Neuroscience

    Background:

    • Action potentials are fundamental to cellular communication in excitable cells.
    • Genetic mutations can alter cellular functions, including electrical excitability.
    • Temperature-sensitive mutations provide insights into protein function and cellular processes.

    Purpose of the Study:

    • To investigate the effect of high temperatures on action potential generation in Paramecium aurelia mutants.
    • To understand the genetic basis of temperature-sensitive defects in cellular excitation.
    • To explore the role of gene products in the initiation and maintenance of action potentials.

    Main Methods:

    • Utilized three Paramecium aurelia mutants with genetic lesions at two unlinked loci.

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  • Cultured mutants at high temperatures to observe phenotypic changes.
  • Recorded and analyzed action potentials at room and elevated temperatures.
  • Examined the kinetics of phenotypic changes following temperature shifts.

    • Main Results:

    • Mutants lost the ability to generate action potentials at high temperatures.
    • Action potentials recorded at room temperature were slightly aberrant.
    • Phenotypic changes in excitation were not immediate upon temperature shifts, indicating a complex regulatory mechanism.
    • Demonstrated that excitation relies on multiple gene products, likely membrane proteins.

    Conclusions:

    • Cellular excitation is dependent on numerous gene products, presumed to be membrane proteins.
    • These gene products are susceptible to modification by both conditional and unconditional mutations.
    • Temperature-sensitive mutations affecting action potential generation highlight the complex interplay between genetics, protein function, and environmental factors in cellular excitability.