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Related Experiment Videos

Complement-dependent release of immune complexes from the lymphocyte membrane.

G W Miller, P H Saluk, V Nussenzweig

    The Journal of Experimental Medicine
    |September 1, 1973
    PubMed
    Summary

    Mouse B lymphocytes rapidly release antigen-antibody-complement complexes via the complement alternate pathway. This serum-mediated release offers a novel assay for alternate pathway function in various species.

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    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • Soluble antigen-antibody-complement complexes can bind to B lymphocytes.
    • The fate and release mechanisms of these complexes from B cells are not fully understood.

    Purpose of the Study:

    • To investigate the mechanism and pathway dependency of antigen-antibody-complement complex release from mouse B lymphocytes.
    • To explore the potential of this release phenomenon as an assay for complement alternate pathway activity.

    Main Methods:

    • Incubation of mouse B lymphocytes with antigen-antibody-complement complexes.
    • Treatment with normal mammalian serum (including C4-deficient serum) and analysis of complex release.
    • Assessment of complement component involvement (Mg(++), Ca(++), C3 proactivator, C3 inactivator).

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    Main Results:

    • Serum rapidly releases bound complexes from B lymphocytes without dissociating or degrading them.
    • Released complexes are altered and cannot rebind to fresh lymphocytes.
    • Release is complement-dependent, preferentially utilizing the alternate pathway (Mg(++) dependent, C4-deficient serum compatible, C3 proactivator required).
    • Serum release activity is heat-labile at 37°C.

    Conclusions:

    • Complement-mediated release of antigen-antibody-complement complexes from B lymphocytes is primarily driven by the alternate pathway.
    • This process involves alteration of the complexes, preventing re-binding.
    • The serum-mediated release serves as a functional assay for the mouse complement alternate pathway and potentially other species.