A method for the determination of amodiaquine

Area of Science:

• Clinical Chemistry
• Analytical Chemistry
• Pharmacology

Background:

• Accurate quantification of amodiaquine is crucial for therapeutic drug monitoring.
• Existing analytical methods may have limitations in sensitivity or practicality.

Purpose of the Study:

• To develop and validate a novel fluorometric assay for amodiaquine analysis.
• To assess the assay's performance in biological matrices like serum, plasma, and red cells.

Main Methods:

• Amodiaquine extraction from alkalinized biological fluid into 1,2-dichloroethane.
• Re-extraction into 0.1 N hydrochloric acid, followed by borate buffer addition.
• Heat-induced fluorescence enhancement of amodiaquine for fluorometric measurement.

Main Results:

• The assay demonstrated linear standard curves between 50 and 3,000 mug/litre in serum and red cells.
• Satisfactory reproducibility and recovery of amodiaquine were achieved.
• Representative amodiaquine levels were reported post-administration in human subjects.

Conclusions:

• The described fluorometric method provides a reliable and reproducible means for amodiaquine quantification.
• This assay is suitable for determining amodiaquine concentrations in clinical and research settings.
• Further studies are needed to confirm assay specificity and characterize the fluorophor.