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Related Experiment Videos

Axons: isolation from mammalian central nervous system.

G H DeVries, W T Norton, C S Raine

    Science (New York, N.Y.)
    |March 24, 1972
    PubMed
    Summary

    Researchers isolated pure axons from white matter. This method removes myelin, yielding axon preparations suitable for studying axonal components like neurofilaments and mitochondria.

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Background:

    • White matter consists of myelinated axons, crucial for nerve impulse transmission.
    • Understanding axonal components requires pure axon preparations, free from myelin sheath.

    Purpose of the Study:

    • To develop a method for isolating pure axons from white matter.
    • To characterize the biochemical and structural components of isolated axons.

    Main Methods:

    • Homogenization of white matter in buffered sucrose solution.
    • Separation of myelinated axons via centrifugation.
    • Swelling and stripping of myelin in hypotonic buffer.
    • Final isolation of axons through differential centrifugation.

    Main Results:

    • A distinct layer of myelinated axons was obtained.
    • Myelin was effectively removed, yielding axon preparations.
    • Isolated axons ranged from 0.3-5.0 micrometers in diameter and up to 200 micrometers in length.
    • Axons contained neurofilaments and mitochondria, but lacked axolemma and neurotubules.
    • The preparation was rich in cerebroside and sulfatide, with minimal myelin contamination.

    Conclusions:

    • This centrifugation-based method successfully isolates pure axons from white matter.
    • The resulting axon preparations are suitable for investigating axonal structures and biochemical composition.
    • The absence of myelin and presence of specific organelles provide insights into axonal integrity.

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