Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Bacteriophage T7 DNA replication: a linear replicating intermediate (gradient centrifugation-electron microscopy-E.

J Wolfson, D Dressler, M Magazin

Proceedings of the National Academy of Sciences of the United States of America

|February 1, 1972

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Variability in feeding practices by parent and eating occasion: A cluster analysis approach.

Appetite·2025

Same author

New formulation of Botox: complete antibody-induced treatment failure in cervical dystonia.

BMJ case reports·2025

Same author

Leveraging ecological momentary assessment to understand variability in food parenting practices within a low-income racially/ethnically diverse sample of parents of preschoolers.

Appetite·2023

Same author

A descriptive assessment of a broad range of food-related parenting practices in a diverse cohort of parents of preschoolers using the novel Real-Time Parent Feeding Practices Survey.

The international journal of behavioral nutrition and physical activity·2022

Same author

COVID-19 pandemic shifts in food-related parenting practices within an ethnically/racially and socioeconomically diverse sample of families of preschool-aged children.

Appetite·2021

Same author

Association of Motor and Cognitive Symptoms with Health-Related Quality of Life and Caregiver Burden in a German Cohort of Advanced Parkinson's Disease Patients.

Parkinson's disease·2020

The T7 bacteriophage chromosome replicates as a Y-shaped DNA rod, unlike previously studied circular bacterial and viral chromosomes. This unique structure challenges existing models of DNA replication.

Area of Science:

Molecular Biology
Virology
Genetics

Background:

Bacterial and viral chromosomes typically replicate as circular DNA molecules.
Understanding DNA replication mechanisms is crucial for molecular biology and virology.

Purpose of the Study:

To investigate the structural dynamics of the T7 bacteriophage chromosome during its initial replication phase.
To compare the replication structure of T7 DNA with known models of bacterial and viral DNA replication.

Main Methods:

Utilizing advanced microscopy techniques to visualize the T7 chromosome.
Employing molecular biology assays to analyze DNA structure during replication.

Main Results:

The T7 chromosome adopts a Y-shaped DNA rod structure during the first round of replication.

Related Experiment Videos

This Y-shaped structure is a distinct departure from the circular forms observed in other systems.

Conclusions:

The T7 bacteriophage exhibits a unique mode of chromosome replication.
The findings necessitate a re-evaluation of DNA replication models, particularly for linear chromosomes.