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Plasmid-determined 2-hydroxypyridine utilization by Arthrobacter crystallopoietes.

M Weinberger, P E Kolenbrander

    Canadian Journal of Microbiology
    |March 1, 1979
    PubMed
    Summary

    Arthrobacter crystallopoietes utilizes 2-hydroxypyridine (2-HP) and produces a blue pigment. This ability is linked to a 63-Mdalton plasmid, which is absent in non-pigment producing strains.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biochemistry

    Background:

    • Arthrobacter crystallopoietes can metabolize 2-hydroxypyridine (2-HP) for carbon and nitrogen.
    • 2-HP metabolism in this bacterium is associated with the production of a blue extracellular pigment.

    Purpose of the Study:

    • To investigate the genetic basis of 2-hydroxypyridine utilization and pigment production in Arthrobacter crystallopoietes.
    • To characterize the plasmid DNA present in pigment-producing (Pig+) and non-pigment-producing (Pig-) strains.

    Main Methods:

    • Ultracentrifugal analysis of cellular extracts from Pig+ and Pig- strains.
    • Plasmid DNA isolation and molecular weight determination.

    Main Results:

    • Both Pig+ and Pig- strains of A. crystallopoietes contain plasmid DNA.
    • Pig+ strains yielded 2-3 times more plasmid DNA than Pig- strains.
    • Plasmid DNA from Pig+ strains had a molecular weight of 62 Mdaltons, slightly higher than Pig- strains.
    • Pig+ strains possess a 63-Mdalton plasmid responsible for 2-HP utilization and pigment formation, along with a cryptic plasmid.
    • Pig- strains contain only the cryptic plasmid.

    Conclusions:

    • The ability of Arthrobacter crystallopoietes to utilize 2-hydroxypyridine and produce a blue pigment is encoded by a specific plasmid.
    • Plasmid content and molecular weight differ between pigment-producing and non-pigment-producing strains, indicating plasmid-borne genetic control.

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