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Related Experiment Videos

Drug-induced erythrocyte membrane internalization.

I Ben-Bassat, K G Bensch, S L Schrier

    The Journal of Clinical Investigation
    |July 1, 1972
    PubMed
    Summary

    Certain drugs, like hydrocortisone, can cause erythrocyte membrane internalization, forming vacuoles. This process is metabolic and can be blocked, suggesting an ATP-dependent mechanism influenced by ion balance.

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    Area of Science:

    • Cell Biology
    • Pharmacology
    • Biochemistry

    Background:

    • Erythrocyte membrane internalization leads to vacuole formation.
    • A radioisotopic method quantifies this process using (37)Co-labeled vitamin B(12).

    Purpose of the Study:

    • To quantify in vitro erythrocyte membrane internalization.
    • To identify drugs that induce this phenomenon.
    • To elucidate the mechanism and influencing factors of membrane internalization.

    Main Methods:

    • Adsorption of a (37)Co-labeled vitamin B(12) complex to erythrocyte surface.
    • Removal of non-internalized label via washing and trypsin treatment.
    • Quantification of residual radioactivity to measure membrane internalization.

    Main Results:

    • Primaquine, hydrocortisone, vinblastine, and chlorpromazine induce erythrocyte membrane internalization.
    • The process is metabolic, dependent on drug concentration, temperature, and pH.
    • Vacuole formation is blocked by substrate depletion or metabolic inhibitors (sodium fluoride, sulfhydryl blockers).

    Conclusions:

    • Membrane internalization is an ATP-energized process influenced by Mg(2+)/Ca(2+) balance.
    • This phenomenon is similar to erythrocyte ghost internalization.
    • Provides a unifying concept for membrane-active drug actions and their role in pathophysiology.

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