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Related Experiment Videos

Heparin interferes with albumin determination by dye-binding methods.

P Bonvicini, G Ceriotti, M Plebani

    Clinical Chemistry
    |August 1, 1979
    PubMed
    Summary

    High concentrations of heparin anticoagulant interfere with albumin testing using bromcresol green dye. This interference is reduced with bromcresol purple and can be eliminated by adding hexadimethrine bromide.

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    Area of Science:

    • Clinical Chemistry
    • Biochemistry

    Background:

    • Albumin is a key protein in human plasma.
    • Accurate albumin determination is crucial for diagnosing various medical conditions.
    • Heparin is a common anticoagulant used in blood collection.

    Purpose of the Study:

    • To investigate the interference of heparin anticoagulant with albumin determination assays.
    • To compare the interference levels between bromcresol green and bromcresol purple methods.
    • To identify methods to mitigate heparin-induced interference in albumin testing.

    Main Methods:

    • In vitro experiments were conducted using varying concentrations of heparin (400-1000 kilounits/L).
    • Albumin levels were measured using the bromcresol green (BCG) and bromcresol purple (BCP) dye-binding methods.
    • The effect of adding hexadimethrine bromide on the interference was assessed.

    Main Results:

    • High concentrations of heparin formed an insoluble precipitate with bromcresol green, causing significant interference in albumin determination.
    • Interference was substantially less pronounced when using bromcresol purple.
    • The addition of hexadimethrine bromide effectively abolished the interference caused by heparin in both BCG and BCP methods.

    Conclusions:

    • Heparin anticoagulant at high concentrations interferes with albumin assays, particularly the bromcresol green method.
    • Bromcresol purple offers reduced interference compared to bromcresol green.
    • Hexadimethrine bromide is a viable agent to eliminate heparin interference in albumin testing.

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