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Updated: Aug 16, 2026

Flat Mount Imaging of Mouse Skin and Its Application to the Analysis of Hair Follicle Patterning and Sensory Axon Morphology
Published on: June 25, 2014
Mesoderm-ectoderm interaction in the production of the agouti pigmentation pattern in mice
Abstract:
Alleles at the agouti locus in the mouse determine the synthesis of either phaeomelanin or eumelanin by follicular melanocytes by altering the hair follicle environment. The method of dermal-epidermal recombination of mouse skin from C57BL/6J a/a and C57BL/6J A(w-J)/A(w-J) embryos was used in this study to establish the precise site of agouti gene action within the hair follicle. The pigmentary pattern of hairs formed in the recombination skin grafts was specific for the genotype of the dermal (mesodermal) component of the hair follicle. The genotype of the epidermal (ectodermal) component had no influence on the type of hair pigmentary pattern. These results indicate that future studies on gene mechanisms should focus on the dermis as the determining factor in altering the hair follicle environment.
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