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Lambda encodes an outer membrane protein: the lom gene.

J N Reeve1, J E Shaw

  • 1Max-Planck-Institut für molekulare Genetik, Abt. Trautner, Berlin.

Molecular & General Genetics : MGG
|January 1, 1979
PubMed
Summary
This summary is machine-generated.

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Lambda-infected minicells produce a specific polypeptide that integrates into the outer membrane. The gene for this lambda outer membrane polypeptide was mapped to a non-essential region of the lambda genome.

Area of Science:

  • Bacteriology
  • Molecular Biology
  • Genetics

Background:

  • Bacteriophage lambda is a model organism for studying gene regulation and DNA replication.
  • Bacteriophage lambda infection can alter host cell physiology, including membrane composition.

Purpose of the Study:

  • To identify and characterize polypeptides synthesized by lambda-infected minicells.
  • To determine the cellular location and genetic origin of a novel lambda-induced polypeptide.

Main Methods:

  • Analysis of protein synthesis in lambda-infected minicells using SDS-polyacrylamide gel electrophoresis.
  • Subcellular fractionation to isolate outer membrane components.
  • Genetic mapping of the gene encoding the polypeptide on the lambda genome.

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Main Results:

  • Lambda-infected minicells synthesized a 20,500 M(r) polypeptide.
  • This polypeptide was predominantly localized to the minicell outer membrane.
  • The gene for this polypeptide, designated lom (lambda outer membrane), was mapped to the 39.4%-40.7% region of the lambda genome.

Conclusions:

  • A novel lambda-induced polypeptide is specifically targeted to the outer membrane of infected minicells.
  • The lom gene resides in a non-essential region of the lambda genome, suggesting its role may be related to modulating host cell envelope properties during infection.