Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mycobacterial agglutination-inhibition test.

W D Richards, W G Eacret

    Applied Microbiology
    |September 1, 1972
    PubMed
    Summary

    The mycobacterial agglutination-inhibition test (MAIT) accurately identifies unstable Mycobacterium avium and Mycobacterium intracellulare isolates. This serological method offers reliable results for mycobacterial identification.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A repository of specimens for comparison of diagnostic testing procedures for bovine paratuberculosis.

    Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·1992
    Same author

    Hearing aid coupler output level variability and coupler correction levels for insert earphones.

    Ear and hearing·1991
    Same author

    Isolation of Mycobacterium kansasii from lymph nodes of cattle in the United States.

    American journal of veterinary research·1983
    Same author

    Preservation of tissue specimens during transport to mycobacteriology laboratories.

    Journal of clinical microbiology·1983
    Same author

    Comment on "a comparative study of alternative bone-conduction calibration methods".

    Ear and hearing·1982
    Same author

    Frequency response and output variations of Radioear B-71 and B-72 bone vibrators.

    Ear and hearing·1982

    Area of Science:

    • Microbiology
    • Immunology
    • Serology

    Background:

    • Standard serological identification methods often exclude Mycobacterium avium isolates due to unstable cell suspensions.
    • Accurate identification of Mycobacterium avium and Mycobacterium intracellulare is crucial for clinical and research purposes.

    Purpose of the Study:

    • To evaluate the mycobacterial agglutination-inhibition test (MAIT) for identifying unstable Mycobacterium avium and Mycobacterium intracellulare isolates.
    • To compare the efficacy and reproducibility of the MAIT method against the mycobacterial agglutination of Schaefer (MATS) and animal pathogenicity tests.

    Main Methods:

    • The mycobacterial agglutination-inhibition test (MAIT), a modification of MATS, was employed.
    • MAIT involved absorption and agglutination tests.
    • 100 unstable M. avium, 3 unstable M. intracellulare, and 20 stable isolates were tested.

    Main Results:

    • The MAIT method successfully identified 100 unstable M. avium and 3 unstable M. intracellulare isolates.
    • MAIT results were comparable to animal pathogenicity tests for unstable isolates.
    • MAIT and MATS yielded identical serological identifications for stable M. avium and M. intracellulare isolates.
    • The reproducibility of MAIT was confirmed through three consecutive tests on 123 cultures.

    Conclusions:

    • The mycobacterial agglutination-inhibition test (MAIT) is a reliable and reproducible method for the serological identification of Mycobacterium avium and Mycobacterium intracellulare, including challenging unstable isolates.
    • MAIT overcomes limitations of previous methods, enabling accurate identification of previously excluded mycobacterial strains.

    Related Experiment Videos