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Updated: Jul 22, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
Published on: November 12, 2012
Bidirectional replication of the chromosome in Escherichia coli
Abstract:
E. coli cells initiating chromosome replication after release from amino-acid starvation were incubated in [(3)H]thymine of moderate specific activity, followed by incubation in [(3)H]thymine plus [(3)H]thymidine of very high specific activity. The grain tracks produced in autoradiographs of chromosomes were denser on both ends than in the middle. The autoradiographic patterns are, therefore, evidence that replication of the chromosome in E. coli is bidirectional.
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