Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Behavior of coliphage lambda in Shigella flexneri 2a.

P Gemski, J A Alexeichik, L S Baron

    Journal of Virology
    |October 1, 1972
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    SEB is cytotoxic and alters EC barrier function through protein tyrosine phosphorylation in vitro.

    The American journal of physiology·1997
    Same author

    Mitogenic activities of amino acid substitution mutants of staphylococcal enterotoxin B in human and mouse lymphocyte cultures.

    Infection and immunity·1996
    Same author

    The importance of a lipopolysaccharide-initiated, cytokine-mediated host defense mechanism in mice against extraintestinally invasive Escherichia coli.

    The Journal of clinical investigation·1995
    Same author

    Identification of staphylococcal enterotoxin B sequences important for induction of lymphocyte proliferation by using synthetic peptide fragments of the toxin.

    Infection and immunity·1994
    Same author

    Method for simultaneous isolation and quantitation of platelet activating factor and multiple arachidonate metabolites from small samples: analysis of effects of Staphylococcus aureus enterotoxin B in mice.

    Analytical biochemistry·1994
    Same author

    Role of Shiga-like toxin I in bacterial enteritis: comparison between isogenic Escherichia coli strains induced in rabbits.

    Gastroenterology·1994
    Same journal

    Something old, something new? Herpesvirus genome packaging examined in light of lessons from the tailed bacteriophages.

    Journal of virology·2026
    Same journal

    Receptor profiling and growth assessment of influenza A virus in porcine mammary and non-mammary tissues and derived cells.

    Journal of virology·2026
    Same journal

    Live human metapneumovirus vaccine candidates attenuated by temperature sensitivity mutations from human respiratory syncytial virus.

    Journal of virology·2026
    Same journal

    Structure and functional analyses of vaccinia virus J5 protein reveal distinct determinants for entry-fusion complex assembly and activation.

    Journal of virology·2026
    Same journal

    Coronavirus membrane protein with a fluorescent protein tag enables particle tracking for the study of virus assembly and egress in live cells.

    Journal of virology·2026
    Same journal

    Disruption of the S1/S2 multibasic cleavage site attenuates infectious bronchitis virus, while S2' partially restores viral virulence and expands tissue tropism.

    Journal of virology·2026
    See all related articles

    Shigella flexneri 2a

    Area of Science:

    • Microbiology
    • Bacteriology
    • Virology

    Background:

    • Wild-type Shigella flexneri 2a exhibits insensitivity to coliphage lambda.
    • This insensitivity is linked to a genetic defect in the malA gene cluster and the bacterium's "smooth" lipopolysaccharide layer, hindering lambda adsorption.

    Purpose of the Study:

    • To investigate the genetic basis of Shigella flexneri 2a's insensitivity to coliphage lambda.
    • To develop functional Shigella flexneri 2a hosts for coliphage lambda propagation and transduction.

    Main Methods:

    • Repairing the malA lesion in Shigella flexneri 2a to enable expression of the malB-lambdarcp region.
    • Introducing mutations to induce a "rough" lipopolysaccharide character in Shigella flexneri.
    • Assessing coliphage lambda adsorption, lysogenization, and transduction capabilities in modified Shigella flexneri hosts.

    Related Experiment Videos

    Main Results:

    • Repairing the malA defect yielded Shigella flexneri derivatives capable of serving as functional hosts for lambda.
    • Mutations causing a "rough" lipopolysaccharide phenotype enhanced lambda adsorption.
    • Lambda propagated on a malA(+) rough Shigella flexneri host showed specific restriction patterns in Escherichia coli strains.

    Conclusions:

    • The malA gene cluster and lipopolysaccharide structure are critical determinants of Shigella flexneri 2a's susceptibility to coliphage lambda.
    • Modified Shigella flexneri strains can serve as effective hosts for lambda propagation and yield gal(+)-transducing lysates.