Standardization and quantitation of immunofluorescence in the rabies fluorescent-antibody test

The standardization and quantitation of immunofluorescence with a Farrand microphotometer in the rabies fluorescent-antibody technique were determined. The system is useful in maintaining the quality of examinations for rabies in a public health laboratory since quantitation of reagent, equipment performance, and specificity of reaction can be evaluated. An analysis of the results demonstrates the high degree of reproducibility and the accuracy of the techniques described.