Comparative immunogenicity of heat-killed and living oral Salmonella vaccines

CD-1 mice were vaccinated intragastrically or intramuscularly with one or two doses of 200 mug of heat-killed Salmonella enteritidis 5694. Control mice were vaccinated with sublethal doses of living S. enteritidis Se795. The mice were challenged intragastrically with approximately 10(6)S. enteritidis 5694 SM(R) 7 to 14 days later, and the growth of the challenge population in the liver, spleen, mesenteric lymph nodes, lungs, and intestine was measured quantitatively. Mice receiving two doses of heat-killed vaccine by mouth were able to delay the systemic emergence of a gastrically introduced salmonella infection by 1 to 2 days. The corresponding liver and spleen populations were slightly lower than those seen in the normal controls. On the other hand, mice receiving the living, attenuated vaccine (either intravenously or intragastrically) developed an effective anti-salmonella immunity against subsequent reinfection.