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Binucleate classical mycoplasmas pathogenic for goats.

G Furness, M D De Maggio

    Infection and Immunity
    |April 1, 1972
    PubMed
    Summary
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    Pathogenic goat mycoplasmas, including Mycoplasma mycoides, were studied for growth and survival. Findings reveal their sensitivity to storage, freezing, and filtration, with replication occurring via budding.

    Area of Science:

    • Veterinary Microbiology
    • Mycoplasma Research
    • Bacterial Physiology

    Background:

    • Goat mycoplasmas are significant pathogens in livestock.
    • Understanding their growth characteristics is crucial for disease control.

    Purpose of the Study:

    • To characterize the growth and survival of three pathogenic goat mycoplasma strains.
    • To investigate their response to various environmental conditions and physical treatments.

    Main Methods:

    • Cultivation on thallium acetate agar.
    • Assessment of inactivation by storage, freezing, thawing, shaking, and sonic treatment.
    • Ultraviolet inactivation studies.
    • Membrane filtration experiments (0.45, 0.3, and 0.22 μm).
    • Electron microscopy for cell morphology and replication analysis.

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    Main Results:

    • All strains formed classical colonies on selective agar.
    • Mycoplasmas were inactivated by cold storage, freezing/thawing, but not shaking.
    • Strain KH1 was susceptible to sonic treatment.
    • Ultraviolet inactivation indicated single binucleate cells.
    • Filtration effectively removed mycoplasmas, with minimal passage through 0.22 μm filters.
    • Electron microscopy revealed replication by budding, with cells ranging from 0.1 to 0.9 μm, often undergoing binary fission or simultaneous nuclear division.

    Conclusions:

    • Pathogenic goat mycoplasmas exhibit distinct growth and inactivation profiles.
    • Replication occurs through budding and binary fission, with evidence of synchronized nuclear division.
    • Filtration is an effective method for removing these mycoplasmas from cultures.