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Pseudomonas putrefaciens isolates from clinical specimens.

P S Riley, H W Tatum, R E Weaver

    Applied Microbiology
    |November 1, 1972
    PubMed
    Summary
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    This study differentiated Pseudomonas putrefaciens clinical isolates into two groups based on growth characteristics and carbohydrate fermentation. Group 1 showed salt sensitivity and fermented specific sugars, while Group 2 exhibited salt tolerance and limited fermentation.

    Area of Science:

    • Microbiology
    • Clinical Microbiology

    Background:

    • Pseudomonas putrefaciens is a bacterial species found in clinical specimens.
    • Accurate identification and differentiation of bacterial isolates are crucial for effective clinical management.

    Purpose of the Study:

    • To characterize and differentiate 109 clinical isolates of Pseudomonas putrefaciens.
    • To establish distinct phenotypic profiles for Pseudomonas putrefaciens subgroups.

    Main Methods:

    • Isolation and cultivation of Pseudomonas putrefaciens from clinical samples.
    • Assessment of growth in nutrient broth with varying NaCl concentrations.
    • Evaluation of growth on Salmonella-Shigella (SS) agar.
    • Carbohydrate fermentation profiling using various sugars.

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    Main Results:

    • Two distinct groups of isolates were identified based on phenotypic characteristics.
    • Group 1 (31 isolates) demonstrated salt sensitivity (no growth above 7% NaCl), no growth on SS agar, and acid production from sucrose, maltose, arabinose, and dextrin.
    • Group 2 (78 isolates) exhibited salt tolerance (growth at 7-10% NaCl), growth on SS agar, and limited acid production, primarily variable with glucose and fructose.

    Conclusions:

    • Phenotypic characterization, including salt tolerance and carbohydrate fermentation, effectively differentiates clinical isolates of Pseudomonas putrefaciens.
    • The identified groups represent distinct subpopulations within Pseudomonas putrefaciens with differing environmental and growth requirements.