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Updated: Aug 10, 2026

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis
Published on: December 8, 2011
Genital yeast infections
Abstract:
Genital yeast infection was studied in 533 women seen in a department of venereology. Yeasts were recovered in culture from 138 patients (26% of the total). Candida albicans accounted for 112 (81%) of the isolates and Torulopsis glabrata for 22 (16%); other yeasts were uncommon. There was no evidence that the presence of yeasts was related to age. 32% of the women who were taking an oral contraceptive harboured yeasts, compared with 18% of those who were not.The symptoms and signs of the women with yeast infections were compared with those with vaginal trichomoniasis and those with no evidence of genital infection. It seems that a clinical diagnosis of vaginal mycosis cannot be made with accuracy and that positive identification of yeasts is necessary; for this, cultural methods are the most satisfactory.
Insights
Genital yeast infections are common, with Candida albicans being the most frequent cause. Accurate diagnosis requires laboratory identification, as clinical symptoms alone are unreliable for detecting vaginal mycosis.
Area of Science:
- Gynecology
- Mycology
- Infectious Diseases
Background:
- Genital yeast infections are a common condition affecting women.
- Previous studies suggest a potential link between oral contraceptive use and yeast colonization.
- Accurate diagnosis of vaginal mycosis is crucial for effective treatment.
Purpose of the Study:
- To investigate the prevalence and causative agents of genital yeast infections in women.
- To explore the association between oral contraceptive use and yeast carriage.
- To evaluate the diagnostic accuracy of clinical assessment versus laboratory methods for vaginal mycosis.
Main Methods:
- A study involving 533 women attending a venereology department.
- Yeast cultures were performed on all participants to identify causative organisms.
- Clinical symptoms and signs were compared between women with yeast infections, trichomoniasis, and no infection.
Main Results:
- Yeasts were recovered from 26% of the women, with Candida albicans (81%) and Torulopsis glabrata (16%) being the most common isolates.
- No significant relationship was found between yeast presence and age.
- Women using oral contraceptives showed a higher prevalence of yeast carriage (32%) compared to non-users (18%).
Conclusions:
- Clinical diagnosis of vaginal mycosis is often inaccurate.
- Laboratory identification of yeasts through cultural methods is essential for accurate diagnosis.
- Oral contraceptive use may be associated with an increased risk of genital yeast colonization.
Related Concept Videos
Yeast Signaling
Sexually Transmitted Infections
Microbiota of the Urogenital Tract
Genital Herpes
Candidiasis
Trichomoniasis

