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Updated: Aug 12, 2026

Preparing T Cell Growth Factor from Rat Splenocytes
Published on: October 31, 2007
Stimulation of IgG antibody response in vitro by T cell-replacing factor
Abstract:
A soluble factor (TRF) produced by mixtures of allogeneic mouse spleen, lymph node, and thymus cells functionally replaces T cells in a primary IgM antibody response to sheep blood cells in vitro. It is now shown that TRF can also reconstitute an IgG antibody response in T cell-deprived spleen cultures derived from preimmunized mice. The optimal time of addition and the amount of TRF required differ between primary and secondary in vitro systems.
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