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Updated: Aug 17, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
Regulation of the expression of the N gene of bacteriophage lambda
Abstract:
A quantitative assay for the N protein of bacteriophage lambda has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free protein-synthesizing system from Escherichia coli programmed with lambdaN(-) DNA the lambda endolysin is made only if N protein is added to the reaction. The rate of synthesis of N protein in vivo is negatively controlled by the products of the CI and tof genes of the phage. Furthermore, N protein activity is extremely unstable in vivo. During normal cell growth at 35 degrees , the half-life of N protein is about 2 min.
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