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Treponeme outer cell envelope: solubilization and reaggregation.

R C Johnson, M S Wachter, D M Ritzi

    Infection and Immunity
    |February 1, 1973
    PubMed
    Summary
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    Low concentrations of sodium dodecyl sulfate solubilize the outer envelope of the avirulent Kazan 5 treponeme. Cations are crucial for reaggregation, influencing yield and structure, with ethylenediaminetetraacetic acid or sodium dodecyl sulfate enabling resolubilization.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Cell Biology

    Background:

    • The avirulent Kazan 5 treponeme is a bacterial species relevant in microbiological studies.
    • Understanding the structural components of treponemes, particularly their outer envelope, is essential for characterizing these organisms.
    • Sodium dodecyl sulfate is a common detergent used in biochemical analyses.

    Purpose of the Study:

    • To investigate the effect of sodium dodecyl sulfate on the outer envelope of the avirulent Kazan 5 treponeme.
    • To determine the role of cations in the reaggregation of the treponeme outer envelope.
    • To explore methods for resolubilizing the reaggregated outer envelope preparation.

    Main Methods:

    • Exposure of treponeme cells to specific concentrations of sodium dodecyl sulfate.

    Related Experiment Videos

  • Analysis of outer envelope solubilization and reaggregation.
  • Investigation of cation concentration effects on aggregate yield and structure.
  • Use of ethylenediaminetetraacetic acid and sodium dodecyl sulfate for resolubilization.

    • Main Results:

    • Low concentrations (1.4 mm) of sodium dodecyl sulfate effectively solubilize the outer envelope of the Kazan 5 treponeme.
    • Cations are required for maximal reaggregation of the solubilized outer envelope.
    • The concentration of cations influences both the yield and structural integrity of the reaggregated preparation.
    • The reaggregated outer envelope can be resolubilized using ethylenediaminetetraacetic acid or sodium dodecyl sulfate.

    Conclusions:

    • Sodium dodecyl sulfate is effective in disrupting and solubilizing the treponeme outer envelope.
    • Cation-dependent reaggregation provides insights into the structural organization of the outer envelope.
    • The ability to resolubilize the preparation offers potential for further biochemical analysis.