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An antiphagocytic factor associated with group E streptococcus.

R A Daynes, C H Armstrong

    Infection and Immunity
    |February 1, 1973
    PubMed
    Summary
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    Group E streptococci (GES) grown in serum exhibit resistance to phagocytosis due to an antiphagocytic factor (APF). This APF, linked to pathogenicity, is associated with a fimbriated surface component.

    Area of Science:

    • Microbiology
    • Immunology
    • Veterinary Science

    Background:

    • Group E streptococci (GES) are a significant cause of streptococcic lymphadenitis (SLS) in swine.
    • Understanding the mechanisms of GES pathogenicity is crucial for developing effective vaccines and treatments.

    Purpose of the Study:

    • To investigate the antiphagocytic properties of GES grown under different conditions.
    • To identify and characterize the factor responsible for resistance to phagocytosis.
    • To explore the role of this factor in GES pathogenicity and immune response.

    Main Methods:

    • Culturing GES in Tryptose phosphate broth (TPB) with and without serum supplementation.
    • Assessing phagocytosis susceptibility using porcine leukocytes.
    • Characterizing the antiphagocytic factor (APF) through enzymatic digestion (trypsin) and electron microscopy.

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  • Testing the neutralization of APF by immune swine sera.

    • Main Results:

    • GES grown in serum-supplemented TPB (STPB) were resistant to nonspecific phagocytosis, unlike those grown in TPB.
    • Serum supplementation induced the elaboration of an antiphagocytic factor (APF) distinct from polysaccharide antigens.
    • Electron microscopy revealed a fimbriated surface component on STPB-grown GES, absent in TPB-grown GES.
    • APF was trypsin-labile and specifically neutralized by sera from swine immune to SLS.

    Conclusions:

    • Serum supplementation induces an antiphagocytic factor (APF) in GES, likely contributing to pathogenicity.
    • The APF is associated with a fimbriated surface component, potentially analogous to M protein.
    • The APF plays a role in the induction of protective immunity against streptococcic lymphadenitis (SLS).