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Related Experiment Videos

Extrachromosomal deoxyribonucleic acid in different enterobacteria.

C Christiansen, G Christiansen, A L Bak

    Journal of Bacteriology
    |April 1, 1973
    PubMed
    Summary

    Extrachromosomal deoxyribonucleic acid (DNA) was found in 34 of 87 bacterial strains, primarily in Escherichia and Klebsiella. These circular DNA molecules varied in size and density, indicating diverse extrachromosomal elements in bacteria.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Extrachromosomal deoxyribonucleic acid (DNA) plays a crucial role in bacterial adaptation and evolution.
    • Understanding the prevalence and characteristics of extrachromosomal DNA in common bacterial genera is essential.

    Purpose of the Study:

    • To investigate the presence and characteristics of extrachromosomal DNA in enterobacteria and Pseudomonas strains.
    • To determine the frequency, size, and density of extrachromosomal DNA in these bacterial groups.

    Main Methods:

    • Analysis of 87 bacterial strains including enterobacteria and Pseudomonas.
    • Detection of extrachromosomal DNA using ethidium bromide CsCl density gradient centrifugation.
    • Characterization of isolated DNA via analytical ultracentrifugation and electron microscopy.

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    Main Results:

    • Thirty-four strains (39%) harbored extrachromosomal DNA, most frequently observed in Escherichia and Klebsiella.
    • Circular DNA molecules ranged from small (0.5-4 µm) to large (20-40 µm) sizes.
    • Buoyant densities varied from 1.692 to 1.721 g/cm³, with some strains containing multiple density classes.

    Conclusions:

    • Extrachromosomal DNA is prevalent in the examined bacterial populations, particularly in Escherichia and Klebsiella.
    • The diverse size and density profiles suggest a variety of extrachromosomal elements, potentially including plasmids.
    • Further research into the functional significance of these extrachromosomal DNA elements is warranted.