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Related Experiment Videos

Delayed-incubation membrane-filter test for fecal coliforms.

R H Taylor, R H Bordner, P V Scarpino

    Applied Microbiology
    |March 1, 1973
    PubMed
    Summary

    A new delayed-incubation method for fecal coliforms closely matches immediate test results in freshwater. This technique offers flexibility for water quality monitoring when immediate testing isn't feasible.

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    Area of Science:

    • Environmental microbiology
    • Water quality analysis
    • Microbiological methods

    Background:

    • Standard Methods for the Examination of Water and Wastewater (13th ed., 1971) outlines an immediate fecal coliform test.
    • Timely analysis of fecal coliforms is crucial for assessing water safety.
    • Sample storage and transport limitations can hinder immediate testing.

    Purpose of the Study:

    • To develop and evaluate a delayed-incubation membrane-filter technique for fecal coliform enumeration.
    • To compare the accuracy of the delayed-incubation method with the standard immediate test.
    • To assess the applicability of the new method across different water types.

    Main Methods:

    • A novel delayed-incubation membrane-filter technique was developed using a vitamin-free Casitone holding medium.
    • Laboratory and field evaluations were conducted comparing the delayed-incubation test with the immediate fecal coliform test.
    • The method's efficacy was tested on various freshwater samples and limited saline water samples.

    Main Results:

    • The delayed-incubation test demonstrated fecal coliform counts closely approximating those of the immediate test in freshwater samples.
    • The method proved effective regardless of freshwater source or type.
    • The technique showed reduced effectiveness with saline water samples.

    Conclusions:

    • The developed delayed-incubation membrane-filter technique provides a reliable alternative for fecal coliform testing in freshwater.
    • This method is particularly valuable for survey monitoring and emergency situations where immediate testing is impractical.
    • The procedure offers a standardized approach for remote laboratory analysis of water and waste discharges.

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