Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualization of freezing damage.

H Bank, P Mazur

    The Journal of Cell Biology
    |June 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Cell survival during freezing depends on cooling rate. Rapid cooling causes intracellular ice, while slow cooling leads to solution effects, impacting yeast cell viability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessing physical activity in people with mental illness: 23-country reliability and validity of the simple physical activity questionnaire (SIMPAQ).

    BMC psychiatry·2020
    Same author

    A complex four-point method for the evaluation of ohmic and faradaic losses within a redox flow battery single-cell.

    MethodsX·2019
    Same author

    Dehydration Preparation of Mouse Sperm for Vitrification and Rapid Laser Warming.

    Cryo letters·2016
    Same author

    Factors influencing quality of anticoagulation control and warfarin dosage in patients after aortic valve replacement within the 3 months of follow up.

    Journal of physiology and pharmacology : an official journal of the Polish Physiological Society·2016
    Same author

    Influence of the surface properties on bactericidal and fungicidal activity of magnetron sputtered Ti-Ag and Nb-Ag thin films.

    Materials science & engineering. C, Materials for biological applications·2016
    Same author

    Measurement and comparison of individual external doses of high-school students living in Japan, France, Poland and Belarus-the 'D-shuttle' project.

    Journal of radiological protection : official journal of the Society for Radiological Protection·2015
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Area of Science:

    • Cryobiology
    • Cell Biology
    • Microbiology

    Background:

    • Cellular damage during freezing is influenced by cooling rates.
    • Understanding these effects is crucial for cryopreservation techniques.

    Purpose of the Study:

    • To investigate the relationship between cooling velocity and cell viability in Saccharomyces cerevisiae.
    • To test the hypothesis that intracellular ice and solution effects determine cell survival during freezing.

    Main Methods:

    • Utilized freeze-cleaving and freeze-fracture replication to examine yeast ultrastructure.
    • Cooled yeast cell suspensions at rates from 1.8 to 75,000 °C/min.
    • Minimized artifacts through immediate replication, deep etching, or rewarming/recooling protocols.

    Related Experiment Videos

    Main Results:

    • Yeast cells cooled at or above approximately 7 °C/min (optimal viability rate) exhibited intracellular ice.
    • Cells cooled below this critical rate showed no intracellular ice, suggesting survival is limited by other factors.
    • Freeze-cleaving provided direct ultrastructural evidence of intracellular ice formation.

    Conclusions:

    • Cooling velocity is a critical determinant of cell survival during freezing.
    • Intracellular ice formation at rapid cooling rates and solution effects at slower rates are key factors.
    • These findings offer insights into optimizing cryopreservation strategies for yeast and other cells.