Equal sensitivity of the two strands of phiX174 replicative DNA to breakage by ionizing radiation

The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of energy to a specific location of the DNA molecule. The results indicate that both strands of the double helix are equally broken, even though their base compositions are significantly different. If there are "hot spots" in the strands, then they are present in equal amounts in each strand.