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Conformational changes associated with complex formation between a mycobacterial polymethylpolysaccharide and

K K Yabusaki, R E Cohen, C E Ballou

    The Journal of Biological Chemistry
    |August 10, 1979
    PubMed
    Summary

    Mycobacterium smegmatis polysaccharide forms a complex with palmitic acid, suggesting a coiled structure. This interaction, studied via NMR, reveals the fatty acid

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    Area of Science:

    • Biochemistry and Structural Biology
    • Carbohydrate Chemistry
    • Nuclear Magnetic Resonance Spectroscopy

    Background:

    • Mycobacterium smegmatis 3-O-methylmannose polysaccharide is a complex carbohydrate.
    • Understanding polysaccharide-lipid interactions is crucial for biological systems.

    Purpose of the Study:

    • To investigate the structural conformation of Mycobacterium smegmatis 3-O-methylmannose polysaccharide.
    • To determine the interaction between the polysaccharide and palmitic acid.
    • To elucidate the formation of inclusion complexes between methylated polysaccharides and lipids.

    Main Methods:

    • Nuclear Magnetic Resonance (NMR) spectroscopy was employed.
    • Chemical shifts of anomeric proton resonances were analyzed.
    • The effect of varying palmitic acid concentrations on the polysaccharide structure was observed.

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    Main Results:

    • The polysaccharide exhibited intermediate chemical shifts between nonaglucoamylose and alpha-cyclodextrin.
    • Addition of palmitic acid caused an upfield shift in resonances, indicating a tightened coil formation.
    • The interaction formed an inclusion complex, with chemical shift changes being linear with lipid concentration.

    Conclusions:

    • Mycobacterium smegmatis 3-O-methylmannose polysaccharide possesses a secondary structure.
    • Palmitic acid inclusion induces a conformational change, forming a tightened coil structure.
    • The fatty acid inserts into the polysaccharide coil, with its carboxyl group near the methyl aglycon.