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Concanavalin A-induced endocytosis in rabbit reticulocytes, and its decrease with reticulocyte maturation.

S Zweig, S J Singer

    The Journal of Cell Biology
    |February 1, 1979
    PubMed
    Summary
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    Rabbit reticulocytes, but not mature erythrocytes, exhibit limited endocytosis of Concanavalin A (Con A). This receptor-mediated endocytosis diminishes as reticulocytes mature, suggesting membrane domain changes during red blood cell development.

    Area of Science:

    • Cell Biology
    • Membrane Biology
    • Hematology

    Background:

    • Reticulocytes are immature red blood cells containing ribosomes.
    • Mature erythrocytes lack ribosomes and most organelles.
    • Concanavalin A (Con A) is a lectin that binds to specific carbohydrate residues on cell surfaces.

    Purpose of the Study:

    • To investigate the uptake of Concanavalin A (Con A) by rabbit reticulocytes and erythrocytes.
    • To determine if Con A uptake occurs via endocytosis.
    • To examine the relationship between reticulocyte maturation and Con A endocytosis.

    Main Methods:

    • Use of ferritin-labeled Concanavalin A (Con A).
    • Transmission electron microscopy of thin-sectioned, plastic-embedded cells.
    • Assessment of reticulocyte maturation based on ribosome configurations.

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    Main Results:

    • Concanavalin A (Con A) was endocytosed by rabbit reticulocytes to a limited extent.
    • No Con A endocytosis was observed in rabbit erythrocytes.
    • The extent of Con A endocytosis decreased with increasing reticulocyte maturation.

    Conclusions:

    • Reticulocyte membranes contain mobile domains capable of Concanavalin A (Con A) receptor clustering and endocytosis.
    • These mobile membrane domains are gradually eliminated during reticulocyte maturation.
    • The findings support a model of dynamic membrane organization during red blood cell development.