Immunofixation on cellulose acetate: an improved screening method for monoclonal immunoglobulins

A rapid, simple and economical method is described for typing monoclonal immunoglobulins. It is a modification of the immunofixation electrophoresis method and cellulose acetate has been used as a supporing medium. It involves an initial electrophoretic separation followed by an antigen (Ag)-antibody (Ab) reaction 'in situ'. Eight samples can be typed on each 5.7 cm x 10.5 cm strip and only 20 microliter of commercial antiserum are required (about 2.5 microliter per sample). The method permits detection of monoclonal proteins at concentrations as low as 100 ng/microliter in only 60 min.