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Related Experiment Videos

High volume antisera yields from rabbits using plasmapheresis.

H Wohl, L Weekly

    Journal of Immunological Methods
    |May 10, 1979
    PubMed
    Summary

    A straightforward plasmapheresis technique for rabbits allows weekly collection of 75 ml antisera. Antibody titers remained stable during these intervals, validating the procedure for consistent antiserum production.

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    Area of Science:

    • Immunology
    • Veterinary Medicine
    • Laboratory Animal Science

    Background:

    • Antibody production in rabbits is crucial for various research applications.
    • Efficient and repeatable methods for antiserum collection are needed.
    • Plasmapheresis offers a potential solution for large-scale antibody harvesting.

    Purpose of the Study:

    • To describe a simple plasmapheresis procedure for rabbits.
    • To evaluate the feasibility of weekly antisera collection.
    • To assess antibody titer stability over time.

    Main Methods:

    • A simplified plasmapheresis protocol was developed for rabbits.
    • Approximately 75 ml of antisera was collected weekly.
    • Antibody titers were monitored throughout the collection period.

    Main Results:

    • The described procedure enabled consistent weekly collection of rabbit antisera.
    • Significant antibody titer levels were maintained across weekly intervals.
    • The method proved effective for sustained antisera yield.

    Conclusions:

    • The developed plasmapheresis technique is a simple and effective method for obtaining rabbit antisera.
    • This procedure supports regular antibody harvesting without compromising titer.
    • It provides a reliable source of antisera for research purposes.

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