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Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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High volume antisera yields from rabbits using plasmapheresis

H Wohl, L Weekly

    Journal of Immunological Methods
    |May 10, 1979
    PubMed

    Abstract:

    A simple procedure for plasmapheresis of rabbits is described, permitting collection of approximately 75 ml of antisera to be obtained at weekly intervals. Evidence for maintenance of antibody titer throughout this interval is presented.

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    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...

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