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Procion brown: an intracellular dye for light and electron microscopy
Science (New York, N.Y.)
|December 21, 1973
Summary
Procion brown dye effectively labels lamprey giant interneurons for microscopy. This method avoids harsh chemical treatments, preserving neural tissue integrity.
Area of Science:
- Neuroscience
- Cell Biology
- Biochemistry
Background:
- Lamprey giant interneurons are crucial for motor control research.
- Traditional neural tracing methods like cobalt chloride have limitations.
Purpose of the Study:
- To evaluate Procion brown as a novel dye for injecting lamprey giant interneurons.
- To compare Procion brown with cobalt chloride for neural tracing efficacy and tissue preservation.
Main Methods:
- Iontophoresis was used to inject Procion brown into lamprey giant interneurons.
- The dye's opacity to light and electrons was assessed.
- The preservation of surrounding neuropil integrity was examined post-injection.
Main Results:
- Procion brown successfully labeled lamprey giant interneurons with a pink in situ color.
- The dye is opaque to both light and electron microscopy.
- Elimination of post-injection ammonium sulfide treatment was achieved, preserving neuropil integrity.
Conclusions:
- Procion brown offers an improved method for labeling lamprey giant interneurons.
- This technique enhances neural tracing by simplifying procedures and maintaining tissue structure.