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Related Experiment Videos

The lambda F mutants belong to two cistrons.

C E Boklage, E C Wong, V C Bode

    Genetics
    |October 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Mutations in bacteriophage lambda head gene F reveal two distinct mutant classes. One class produces abnormal phage heads, while the other is defective in head formation, highlighting gene F

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • Bacteriophage lambda head gene F is crucial for forming infectious phage particles.
    • Previous studies identified seven amber mutant sites within gene F.
    • These mutants display varied effects on head formation and DNA packaging.

    Purpose of the Study:

    • To characterize the distinct phenotypes of amber mutants in bacteriophage lambda head gene F.
    • To elucidate the functional roles of different regions within gene F.
    • To understand the complementation interactions between various head gene F mutants.

    Main Methods:

    • Analysis of amber mutants in bacteriophage lambda head gene F.
    • Phenotypic characterization of head particle formation and DNA content.

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  • Complementation assays using crude lysates of different mutant strains.

    • Main Results:

    • Mutant F423 (right half of gene F) produces defective but normally sized head particles containing lambdaDNA, and is complemented by other head gene mutants.
    • Mutants F785, F471, and F730 (left half of gene F) fail to produce headlike particles and are not complemented by other head mutants.
    • The left-half mutants' lysates complement F423's defective particles, and in vivo, F423 complements the left-half mutants, but not vice versa.

    Conclusions:

    • Gene F exhibits at least two functionally distinct regions involved in bacteriophage head assembly.
    • The right half of gene F is involved in head maturation or completion, while the left half is essential for initial head structure formation.
    • Complementation data suggests a sequential or interdependent process in head gene F function.