Characterization of aminoacyl transfer ribonucleic acid formation stimulated by polyamines

From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg(2+), it is shown that both reactions have the same requirements for adenosine triphosphate, tRNA, and aminoacyl-tRNA synthetase. Reaction kinetics are similar in both reactions. The same tRNA is aminoacylated in the presence of either spermine of Mg(2+). Aminoacyl-tRNA formed in the presence of polyamines can be a donor of amino acids in polypeptide synthesis.