Related Experiment Video
Updated: Aug 12, 2026
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
Published on: April 4, 2016
Effect of tetanolysin on platelets and lysosomes
Infection and Immunity
|April 1, 1974
Summary
Tetanolysin, a toxin from tetanus, lyses human and rabbit platelets and liver cell components. This activity, neutralized by antitoxin, suggests tetanolysin
Area of Science:
- Microbiology
- Toxicology
- Cell Biology
Background:
- Tetanus is caused by Clostridium tetani.
- The specific mechanisms of tetanus pathogenesis are not fully understood.
- Bacterial toxins can affect cellular and subcellular structures.
Purpose of the Study:
- To investigate the lytic effects of tetanolysin on platelets and liver cell fractions.
- To determine if tetanolysin contributes to the pathogenesis of tetanus.
Main Methods:
- Partially purified tetanolysin was used.
- Platelet lysis was assessed by optical density and release of serotonin, enzymes, and protein.
- Lysis of liver large granule fraction was assessed by hydrolytic enzyme release.
- Neutralization by antitoxin was tested.
Main Results:
- Tetanolysin caused lysis of human and rabbit platelets.
- Tetanolysin induced the release of hydrolytic enzymes from rabbit liver lysosomes.
- The lytic activity of tetanolysin was neutralized by antitoxin.
Conclusions:
- Tetanolysin exhibits lytic activity against platelets and lysosomal membranes.
- Tetanolysin may play a role in the pathogenesis of clinical tetanus manifestations.
