Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Translational control induced by bacteriophage T7.

P Herrlich, H J Rahmsdorf, S H Pai

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Significance of the ratio of reticulocyte subpopulations in bone marrow and peripheral blood from patients with myelodysplastic syndromes.

    Annals of hematology·2003
    Same author

    Antagonistic signaling pathways regulate alternative splicing of CD44 in T cells.

    Annals of the New York Academy of Sciences·2002
    Same author

    Tissue plasminogen activator levels change with plasma fibrinogen concentrations during pregnancy.

    Annals of hematology·2002
    Same author

    The anti-inflammatory action of glucocorticoid hormones.

    Ernst Schering Research Foundation workshop·2002
    Same author

    Redox regulation in mammalian signal transduction.

    IUBMB life·2002
    Same author

    Iron deficiency anemia increases nitric oxide production in healthy adolescents.

    Annals of hematology·2002

    Bacteriophage T7 uses a translational repressor protein to halt host gene expression, specifically inhibiting beta-galactosidase and S-adenosylmethionine hydrolase synthesis without affecting T7 enzyme production.

    Area of Science:

    • Molecular Biology
    • Virology
    • Bacteriology

    Background:

    • Bacteriophage T7 employs sophisticated mechanisms to control host gene expression during infection.
    • Understanding these mechanisms is crucial for deciphering viral-host interactions and gene regulation.

    Purpose of the Study:

    • To investigate the role of the T7 translational repressor in inhibiting host gene expression.
    • To characterize the specificity and mechanism of action of the T7 translational repressor.

    Main Methods:

    • In vivo and in vitro assays were used to measure enzyme synthesis.
    • Purification of the T7 translational repressor in conjunction with initiation factors.

    Main Results:

    • The T7 translational repressor specifically inhibits the synthesis of host enzymes like beta-galactosidase (EC 3.2.1.23) and T3 S-adenosylmethionine hydrolase (EC 3.3.1.-).

    Related Experiment Videos

  • This inhibition occurs at the translational level and does not affect the synthesis of T7-specific enzymes.
  • The repressor associates with translation initiation factors, suggesting interaction with the host translation machinery.

    • Conclusions:

    • The T7 translational repressor is a key viral factor that selectively shuts down host protein synthesis.
    • Its interaction with translation initiation factors highlights a targeted strategy for viral gene expression control.
    • The proximity of the repressor gene to the Escherichia coli RNA polymerase promoter may facilitate its expression during infection.