Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Double-length, circular, single-stranded DNA from filamentous phage.

F C Wheeler, R H Benzinger, H Bujard

    Journal of Virology
    |September 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Liver-specific expression of interferon gamma following adenoviral gene transfer controls hepatitis B virus replication in mice.

    Gene therapy·2005
    Same author

    Gene therapy progress and prospects: transcription regulatory systems.

    Gene therapy·2004
    Same author

    Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo.

    Gene therapy·2003
    Same author

    Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system.

    Gene therapy·2002
    Same author

    Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector.

    Gene therapy·2002
    Same author

    Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii.

    Nucleic acids research·2001
    Same journal

    Something old, something new? Herpesvirus genome packaging examined in light of lessons from the tailed bacteriophages.

    Journal of virology·2026
    Same journal

    Receptor profiling and growth assessment of influenza A virus in porcine mammary and non-mammary tissues and derived cells.

    Journal of virology·2026
    Same journal

    Live human metapneumovirus vaccine candidates attenuated by temperature sensitivity mutations from human respiratory syncytial virus.

    Journal of virology·2026
    Same journal

    Structure and functional analyses of vaccinia virus J5 protein reveal distinct determinants for entry-fusion complex assembly and activation.

    Journal of virology·2026
    Same journal

    Coronavirus membrane protein with a fluorescent protein tag enables particle tracking for the study of virus assembly and egress in live cells.

    Journal of virology·2026
    Same journal

    Disruption of the S1/S2 multibasic cleavage site attenuates infectious bronchitis virus, while S2' partially restores viral virulence and expands tissue tropism.

    Journal of virology·2026
    See all related articles

    Researchers found double-length circular DNA molecules in filamentous phage particles. These molecules, representing about 0.03% of the total DNA, were purified and characterized, confirming their origin from the phage.

    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Filamentous phage are single-stranded DNA viruses.
    • Previous studies have investigated the structure and DNA content of these phages.

    Purpose of the Study:

    • To investigate the presence and characteristics of double-length DNA molecules in filamentous phage.
    • To determine the origin and properties of these double-length DNA molecules.

    Main Methods:

    • Purification of wild-type and mutant filamentous phage stocks.
    • DNase treatment and exhaustive purification of phage particles.
    • DNA extraction, followed by electron microscopy and size separation using CsCl density gradients.
    • Characterization of DNA molecules by sedimentation analysis and contour length measurements.

    Related Experiment Videos

    Main Results:

    • Approximately 0.03% of purified phage DNA molecules were identified as circular dimers.
    • A distinct peak of double-length, circular DNA molecules (38S) was observed, sedimenting ahead of the monomer peak (24S).
    • These double-length molecules were purified 65-fold and their contour length was found to be dependent on ionic strength.
    • Artifacts were ruled out, confirming that these double-length molecules originate from phage particles.

    Conclusions:

    • Filamentous phage particles contain a small but significant population of double-length circular DNA molecules.
    • These molecules are a genuine component of the phage, not an artifact of the purification process.
    • The findings contribute to understanding the structural diversity and DNA packaging mechanisms within filamentous phage.